Тест на ингибирование перекрестно-реактивной углеводной детерминанты (CCD) может помочь определить ложноположительный результат для растительного аллергена - SIgE, вызванного CCD
Wenting L; Sun B; Wu Z
Method : Two hundred and thirteen patients, with a doctor ' s diagnosis of allergic rhinitis or asthma, IgE towards at least two common inhaled allergens were recruited. Serum samples were analyzed for IgE against tree mix (willow/poplar/elm Tree), common ragweed, mugwort, humulus scandens, peanut, soy, and cross- reactive carbohydrate determinants (CCD) and specific IgE- binding inhibition experiments were performed.
Results : Among the patients sensitized to multiple allergens, 83 patients (39.0%) were plant allergen sensitization (sIgE positive for any of the above six allergens was defined as plant allergen sensitization, PAS), and 57.8% of PAS patients were positive to CCD- sIgE. PAS subjects were more often sensitized to CCD, known to be crossreactive between grass and seeds. CCD inhibited binding to all pollen and seed allergen by 73% to 100% (Table 1). The highest inhibition rate was obtained for Humulus scandens, followed by mugwort and peanut (both 85.2%), common ragweed (81.5%), soy(80.0%) and tree mix (73.0%). It was surprised to find that all sIgE against to pollen and seeds from 23 PAS patients were turned to negative after CCD inhibition.
Conclusion : CCD was positive in the serum of most plant allergensIgE positive patients in South China. More than 73% plant allergenssIgE were eliminated into negative after CCD inhibition experiment, suggesting majority the plant allergic patients in southern China, particularly the sIgE against peanut, soybean and pollen allergens, were false- positive caused by the CCD interference. CCD inhibition test should be used in clinical diagnosis, which can help to avoid misdiagnosis of plant allergens.